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jacalin agarose  (Thermo Fisher)


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    Structured Review

    Thermo Fisher jacalin agarose
    Jacalin Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/jacalin+agarose/1-AMINO-1-CYCLOHEXANECARBOXYLIC+ACID/pm41723919-130-6-8
    Average 94 stars, based on 1 article reviews
    jacalin agarose - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy
    Article Snippet: .. In , human serum IgA1 was purified by using Pierce Jacalin Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

    Article Title: SARS-CoV-2-Neutralizing Humoral IgA Response Occurs Earlier but Is Modest and Diminishes Faster than IgG Response.
    Article Snippet: IgG fractions were obtained from SARS-CoV-2infected individuals’ sera or plasmas using spin column-based antibody purification kit (Protein G) (Cosmo Bio, Tokyo, Japan). .. IgA fractions were purified from the IgG purification flowthrough using Pierce Jacalin agarose (Thermo Fisher Scientific, Waltham, MA) and eluted in phosphate-buffered saline (PBS) using Zeba spin desalting columns, 40K MWCO (Thermo Fisher Scientific). .. The total human IgG November/December 2022 Volume 10 Issue 6 10.1128/spectrum.02716-22 10 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 31 J an ua ry 2 02 4 by 1 91 .1 83 .2 48 .2 38 . and IgA concentrations were determined using the human IgG enzyme-linked immunoassay (ELISA) kit and human IgA ELISA kit, respectively (abcam, Cambridge, UK).

    Article Title: Glycan-specific IgM is critical for human immunity to Staphylococcus aureus
    Article Snippet: .. For IgA1 purification, a Jacalin agarose (Thermo scientific) column was used, followed by elution with 0.1M Melibiose (Sigma). ..

    Article Title: Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy.
    Article Snippet: .. In Fig. 2C, human serum IgA1 was purified by using Pierce Jacalin Agarose (Thermo Fisher Scientific) according to the manufacturer ’s instructions. ..

    Saline:

    Article Title: SARS-CoV-2-Neutralizing Humoral IgA Response Occurs Earlier but Is Modest and Diminishes Faster than IgG Response.
    Article Snippet: IgG fractions were obtained from SARS-CoV-2infected individuals’ sera or plasmas using spin column-based antibody purification kit (Protein G) (Cosmo Bio, Tokyo, Japan). .. IgA fractions were purified from the IgG purification flowthrough using Pierce Jacalin agarose (Thermo Fisher Scientific, Waltham, MA) and eluted in phosphate-buffered saline (PBS) using Zeba spin desalting columns, 40K MWCO (Thermo Fisher Scientific). .. The total human IgG November/December 2022 Volume 10 Issue 6 10.1128/spectrum.02716-22 10 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/s pe ct ru m o n 31 J an ua ry 2 02 4 by 1 91 .1 83 .2 48 .2 38 . and IgA concentrations were determined using the human IgG enzyme-linked immunoassay (ELISA) kit and human IgA ELISA kit, respectively (abcam, Cambridge, UK).

    other:

    Article Title: Glycan-specific IgM is critical for human immunity to Staphylococcus aureus
    Article Snippet: Jacalin Agarose , ThermoFisher Scientific , Cat # 20395.

    Chromatography:

    Article Title: XBB 1.5 monovalent booster vaccination stimulates oral mucosal and systemic immune responses in healthy adults.
    Article Snippet: .. Before each use, 1 mL of Jacalin agarose (Thermo Scientific, #20395) packed into a 10 mL chromatography column (Bio-Rad #731–1550) was washed with 3 mL of binding buffer (175 mM Tris-HCl, pH 7.5). ..

    Binding Assay:

    Article Title: XBB 1.5 monovalent booster vaccination stimulates oral mucosal and systemic immune responses in healthy adults.
    Article Snippet: .. Before each use, 1 mL of Jacalin agarose (Thermo Scientific, #20395) packed into a 10 mL chromatography column (Bio-Rad #731–1550) was washed with 3 mL of binding buffer (175 mM Tris-HCl, pH 7.5). ..

    Affinity Chromatography:

    Article Title: Lupus IgA1 autoantibodies synergize with IgG to enhance plasmacytoid dendritic cell responses to RNA-containing immune complexes
    Article Snippet: Spectral reading was performed on VersaMax microplate reader (Molecular Devices). .. IgA1 was depleted from SLE donor serum by affinity chromatography using Jacalin agarose (Pierce catalog #20395). ..



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